pcDNA3.1(+) - Hyperactive AID-T7 RNA Polymerase-UGI-T2A-tdTa

LOCUS       pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato               10388 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express TRACE editor together with a tdTomato reporter in 
            pcDNA3.1(+) backbone.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A -...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, 
            Marshall JL, Chen F
  TITLE     Efficient, continuous mutagenesis in human cells using a 
            pseudo-random DNA editor.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 
            10.1038/s41587-019-0331-8.
  PUBMED    31844291
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      866..875
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1675..1686
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4163..4411
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4424..4444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4454..4507
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4514..4523
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(5980..5997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5986..6210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6256..6684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6343..6362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6553..6574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6693..6713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6698..7027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6878..7013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6940..6959
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7094..7888
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7148..7167)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7758..7777
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8062..8183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8099..8118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8153..8172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8232..8248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8232..8248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8245..8267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8256..8272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8280..8310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8325..8346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8463..8480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8634..9221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8714..8733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9392..10252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10015..10034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10253..10357)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa atgccgccac catggacagc ctcttgatga accggaggga
      901 gtttctttac caattcaaaa atgtccgctg ggctaagggt cggcgtgaga cctacctgtg
      961 ctacgtagtg aagaggcgtg acagtgctac atccttttca ctggactttg gttatcttcg
     1021 caataagaac ggctgccacg tggaattgct cttcctccgc tacatctcgg actgggacct
     1081 agaccctggc cgctgctacc gcgtcacctg gttcatctcc tggagcccct gctacgactg
     1141 tgcccgacat gtggccgact ttctgcgagg gaaccccaac ctcagtctga ggatcttcac
     1201 cgcgcgcctc tacttctgtg aggaccgcaa ggctgagccc gaggggctgc ggcggctgca
     1261 ccgcgccggg gtgcaaatag ccatcatgac cttcaaagat tatttttact gctggaatac
     1321 ttttgtagaa aaccacggaa gaactttcaa agcctgggaa gggctgcatg aaaattcagt
     1381 tcgtctctcc agacagcttc ggcgcatcct tttgcccctg tatgaggttg atgacttacg
     1441 agacgcattt cgtactagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga
     1501 aagtaacacc atcaacattg ctaagaacga cttctcagac atagagctcg cggctattcc
     1561 gttcaacacc ctggctgacc actacggcga gagactcgct agggagcagc tggcgttgga
     1621 gcatgaatcc tacgagatgg gcgaggctag gttccgcaag atgttcgagc gacaattgaa
     1681 ggcaggggag gtggcggaca acgctgccgc caagcccctg atcacaacct tgctgcccaa
     1741 aatgatcgcg cggatcaacg attggtttga ggaggttaag gcaaaacggg gcaaacgccc
     1801 gaccgcattt caattcctcc aagaaatcaa gcctgaggct gttgcctaca tcactatcaa
     1861 gacgacactg gcgtgtctca caagcgccga caacaccacc gtgcaagccg tcgccagcgc
     1921 catcgggcgg gcaattgagg atgaggcacg gtttggtagg atccgagacc tggaagcgaa
     1981 gcacttcaag aagaacgtgg aagagcagtt gaacaaacgc gtcggccacg tgtataaaaa
     2041 ggctttcatg caggtggtgg aggccgatat gctcagtaag gggctgcttg ggggggaggc
     2101 gtggtcatcc tggcacaagg aggatagcat tcacgtgggg gtccgatgta tcgagatgct
     2161 gatagagagc accggaatgg tctccctcca tcgccagaac gctggggtcg tagggcagga
     2221 ctccgagact attgagctgg cccccgagta tgccgaagca atcgctacac gcgcaggtgc
     2281 actggctggg ataagcccta tgtttcagcc ctgcgtagtg cctccaaagc catggaccgg
     2341 catcacaggg ggtggctatt gggccaacgg taggcggcct ctggccctgg tacgcacgca
     2401 cagcaagaag gcgctcatgc gctatgaaga cgtttacatg cccgaggttt acaaggcgat
     2461 caatatcgcg cagaacaccg cctggaaaat caataagaag gtgttggcgg tcgcaaacgt
     2521 gattaccaag tggaagcatt gcccagtcga ggacataccc gccatagaac gcgaagagct
     2581 gccgatgaag ccggaagaca ttgatatgaa ccccgaggcc ctcaccgcgt ggaaaagagc
     2641 cgcagccgcc gtatacagga aggataaagc gcgcaagtcc ctacgcataa gcctcgagtt
     2701 tatgctggaa caggccaaca agttcgccaa ccacaaagct atctggttcc cctacaacat
     2761 ggactggaga gggagggtct acgccgtcag catgttcaat ccccagggca acgacatgac
     2821 gaagggcctt ctgacattgg caaaggggaa gcctatcgga aaggaggggt actactggct
     2881 caagatccac ggcgccaact gcgcgggagt ggacaaggtt ccatttcccg agcgaattaa
     2941 gttcatcgag gaaaaccacg aaaacattat ggcgtgcgct aaatcccccc tcgagaacac
     3001 atggtgggcc gagcaagact ccccgttctg ttttttggca ttctgctttg agtacgccgg
     3061 tgtgcagcac catggcctct catacaactg ttccctgccc ctggccttcg acggaagttg
     3121 cagtgggatt caacatttca gcgcaatgtt gcgggacgag gtcggtggca gggccgttaa
     3181 cctgctccct tccgaaacgg tgcaggacat ctacggaatc gtggcaaaaa aggtaaacga
     3241 gatcctgcaa gcggatgcca tcaacgggac ggacaatgag gtcgttacgg tgacagacga
     3301 aaatactggg gaaataagcg aaaaggtcaa gctggggacc aaagcactcg cgggtcagtg
     3361 gctcgcctac ggggtgacac gctccgtcac caagagaagc gtgatgaccc tcgcgtacgg
     3421 ttcaaaagaa ttcgccttcc gccagcaagt gctggaggac accatccagc cggcgattga
     3481 ctccgggaag ggtctcatgt ttacccagcc gaaccaggcc gcagggtaca tggccaaact
     3541 gatctgggaa agcgttagcg tcacagtggt cgccgcggtt gaggcgatga attggctgaa
     3601 gagcgcggca aagctcctcg ccgctgaggt gaaggacaaa aagaccggcg aaatcctgcg
     3661 caagcgctgc gccgtccact gggtcacgcc ggatggattc cccgtctggc aggagtacaa
     3721 gaagcccatc cgaacccggc tcaacttgat gttccttggc cagtttcgcc tgcagcccac
     3781 gataaacacc aacaaagaca gcgagatcga cgcccacaag caggagagcg gcatcgcgcc
     3841 caacttcgtg cacagtcagg acgggtccca tctgcggaaa actgttgtgt gggctcacga
     3901 gaagtacggc attgagagct tcgccctgat acacgacagc ttcgggacca taccagcgga
     3961 cgcagcgaac ctgttcaaag ccgtgcggga aacaatggtc gacacctacg aaagctgcga
     4021 cgtactggca gacttctatg accaattcgc cgaccagctt cacgagtcac agctcgacaa
     4081 gatgcccgct ctgcccgcga aaggcaacct gaatttgcgc gacatccttg agagcgattt
     4141 tgcgttcgcc tctggtggtt ctactaatct gtcagatatt attgaaaagg agaccggtaa
     4201 gcaactggtt atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg
     4261 gaacaagccg gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa
     4321 tgtcatgctt ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga
     4381 tagcaacggt gagaacaaga ttaagatgct ctctggtggt tctcccaaga agaagaggaa
     4441 agtcggatcg ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     4501 tggcccaccg gtcgccacca tggtcagtaa gggcgaggaa gtgatcaaag aattcatgcg
     4561 ctttaaagta agaatggagg gttcaatgaa tgggcacgag tttgagatcg aaggagaggg
     4621 tgagggtaga ccatatgaag gtactcagac ggctaaactt aaagtgacca aaggaggacc
     4681 cctgccattt gcctgggata tcttgtcccc gcaatttatg tacggaagta aggcctacgt
     4741 aaagcaccct gcagacatcc ctgattataa aaaactcagt ttcccggaag gtttcaagtg
     4801 ggaaagggtc atgaactttg aagatggcgg attggttacc gttacgcaag actcttctct
     4861 ccaggatggc acactcatct ataaggtcaa aatgaggggt acaaactttc cgcccgacgg
     4921 gccagtaatg cagaagaaaa caatgggatg ggaggcttct accgaaagac tctacccgcg
     4981 agacggagtg ctcaagggag aaatacatca agcgctgaag ctgaaagacg gagggcacta
     5041 ccttgtcgaa ttcaagacta tatatatggc caaaaaaccc gtccagctgc caggctatta
     5101 ttacgtcgat acaaagctcg acattacctc ccataatgaa gactacacga tagtagaaca
     5161 atacgagcgg tcagagggac gacatcatct ttttctcggg cacggaactg gttccaccgg
     5221 ctccggatct agcggaaccg catcatccga ggacaataac atggctgtga taaaagagtt
     5281 tatgcggttt aaggttcgga tggaaggctc tatgaatggt catgaatttg agatcgaggg
     5341 cgaaggcgaa ggacggccgt acgaaggtac ccaaacggcg aagttgaaag ttaccaaggg
     5401 tggccccttg ccgttcgcat gggacatttt gtcaccacaa ttcatgtacg gttccaaagc
     5461 atacgtgaaa catccggccg atattcccga ttacaagaaa ctcagttttc cagaaggctt
     5521 taagtgggag agggttatga actttgagga cggtggactc gtaactgtaa cccaagatag
     5581 cagcttgcag gatggaacac ttatctacaa agttaagatg agagggacga attttcctcc
     5641 ggatggtcct gttatgcaaa aaaagacgat gggatgggaa gcctcaactg agcggcttta
     5701 ccccagagac ggggtactta agggagagat acatcaagct cttaagctca aggatggtgg
     5761 gcactatctc gttgagttta agaccattta catggctaaa aagccggttc aattgccggg
     5821 ttattattat gtggatacga aacttgatat tacatctcat aacgaagact atactattgt
     5881 ggaacagtat gagagatctg aaggcagaca ccatctcttt ctgtatggta tggacgaact
     5941 gtataagtga tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6001 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6061 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6121 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6181 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6241 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgata
     6481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6601 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6661 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6781 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6841 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6961 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7021 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7081 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7141 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7201 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7261 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7321 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7381 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7441 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7501 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7561 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7621 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7681 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7741 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7801 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7861 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     7921 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8041 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8101 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8161 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9181 gtttgcaagc agcagattac gcgcagaaaa aaggatctca agaagatcct ttgatctttt
     9241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9361 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9421 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9481 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9541 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9601 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9661 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9721 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9781 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9841 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9901 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9961 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10021 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10081 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10141 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10201 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10261 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10321 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10381 ctgacgtc
//